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Published on: September 3, 2016
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Changes in Serum Prestin Concentration After Exposure to Cisplatin
Benjamin Liba1, James Naples, Elizabeth Bezyk
1*University of Connecticut School of Medicine †Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, UConn Health, Farmington, Connecticut.
Summary
Serum prestin levels change over time after cisplatin administration in rodents. These findings suggest prestin may be a biomarker for cisplatin-induced ototoxicity.
Area of Science:
- Ototoxicity research
- Biomarker discovery
- Pharmacology
Background:
- Cisplatin is a common chemotherapy agent.
- Cisplatin can cause ototoxicity, or damage to the ear.
- Outer hair cells are crucial for hearing and express the protein prestin.
Purpose of the Study:
- To investigate temporal changes in serum prestin after low-dose cisplatin administration.
- To compare these changes in rodent models with differing cisplatin sensitivity.
- To evaluate prestin as a potential biomarker for cisplatin ototoxicity.
Main Methods:
- Mice and guinea pigs were treated with cisplatin (8 mg/kg).
- Auditory brainstem responses were measured on Days 1, 3, 7, and 14.
- Serum prestin concentrations were quantified using enzyme-linked immunosorbent assays.
Main Results:
- Auditory brainstem response thresholds showed minimal change in mice but increased in guinea pigs.
- Serum prestin concentrations peaked on Days 3 (mice) and 7 (guinea pigs) post-treatment.
- Prestin levels returned to baseline by Day 14 in both species.
Conclusions:
- A time-dependent pattern of serum prestin change was observed after cisplatin exposure.
- Prestin may serve as a valuable biomarker for detecting cisplatin-induced ototoxicity.
- Comparing resistant and sensitive models provides insights into ototoxicity mechanisms.
