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Related Experiment Video

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Silkworm model for Francisella novicida infection.

Shib Shankar Saha1, Jin Suzuki2, Akihiko Uda3

  • 1The United Graduate School of Veterinary Science, Yamaguchi University, 1677-1, Yoshida, Yamaguchi 753-8515, Japan; Department of Pathology and Parasitology, Patuakhali Science and Technology University, Babugonj, Barisal 8210, Bangladesh.

Microbial Pathogenesis
|October 26, 2017
PubMed
Summary

A novel silkworm infection model effectively demonstrated Francisella novicida pathogenesis. The DotU protein was confirmed as a crucial virulence factor, essential for bacterial survival and host mortality in this insect model.

Keywords:
DotUFrancisella novicidaInfection modelSilkwormVirulence factor

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Insect Pathology

Background:

  • Insect models are valuable for studying human pathogen virulence and pathogenesis.
  • Francisella novicida, a mouse-virulent but human-non-pathogenic bacterium, serves as a key research model.
  • Understanding Francisella virulence factors is crucial for developing effective countermeasures.

Purpose of the Study:

  • To establish and validate a silkworm (Bombyx mori) infection model for Francisella novicida.
  • To investigate the role of the DotU protein in Francisella novicida pathogenesis using the silkworm model.
  • To assess the suitability of the silkworm model for identifying novel bacterial virulence factors.

Main Methods:

  • Inoculation of silkworm hemocoels with wild-type and DotU deletion mutant strains of Francisella novicida.
  • Monitoring silkworm survival rates and quantifying bacterial loads in whole bodies and specific organs.
  • Utilizing confocal microscopy to assess bacterial growth within silkworm hemocytes and the BmN4 cell line.

Main Results:

  • Francisella novicida infection caused silkworm mortality within 3-7 days.
  • A DotU deletion mutant significantly reduced bacterial load and failed to induce mortality, indicating DotU's essential role.
  • Bacterial growth was significantly impaired in the DotU mutant within silkworm hemocytes and the BmN4 cell line.
  • Bacterial load distribution showed variations across different silkworm tissues.

Conclusions:

  • The silkworm (Bombyx mori) serves as a viable and effective infection model for studying Francisella novicida pathogenesis.
  • The DotU protein is a critical virulence factor for Francisella novicida in the silkworm model, mirroring its role in mammalian systems.
  • This silkworm infection model is a promising tool for the discovery and characterization of new bacterial virulence factors.