Muscle oxygenation profiles between active and inactive muscles with nitrate supplementation under hypoxic exercise

Whether dietary nitrate supplementation improves exercise performance or not is still controversial. While redistribution of sufficient oxygen from inactive to active muscles is essential for optimal exercise performance, no study investigated the effects of nitrate supplementation on muscle oxygenation profiles between active and inactive muscles. Nine healthy males performed 25 min of submaximal (heart rate ~140 bpm; EX_sub) and incremental cycling (EX_max) until exhaustion under three conditions: (A) normoxia without drink; (B) hypoxia (FiO₂ = 13.95%) with placebo (PL); and (c) hypoxia with beetroot juice (BR). PL and BR were provided for 4 days. Oxygenated and deoxygenated hemoglobin (HbO₂ and HHb) were measured in vastus lateralis (active) and biceps brachii (inactive) muscles, and the oxygen saturation of skeletal muscle (StO₂; HbO₂/total Hb) were calculated. During EX_sub, BR suppressed the HHb increases in active muscles during the last 5 min of exercise. During EX_max, time to exhaustion with BR (513 ± 24 sec) was significantly longer than with PL (490 ± 39 sec, P < 0.05). In active muscles, BR suppressed the HHb increases at moderate work rates during EX_max compared to PL (P < 0.05). In addition, BR supplementation was associated with greater reductions in HbO₂ and StO₂ at higher work rates in inactive muscles during EX_max Collectively, these findings indicate that short-term dietary nitrate supplementation improved hypoxic exercise tolerance, perhaps, due to suppressed increases in HHb in active muscles at moderate work rates. Moreover, nitrate supplementation caused greater reductions in oxygenation in inactive muscle at higher work rates during hypoxic exercise.