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Published on: July 6, 2016
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Study on the interaction between cannabinol and DNA using acridine orange as a fluorescence probe
Zhengshan Tian1, Zechun Wang2, Xinxin Han2
1School of Chemistry and Environmental Engineering, Pingdingshan University, Pingdingshan, China.
Journal of Molecular Recognition : JMR
|October 26, 2017
Summary
Cannabinol (CBN) binds to herring sperm DNA, as shown by fluorescence quenching experiments. Hydrophobic interactions drive this binding, confirmed by molecular docking and thermodynamic analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- Cannabinol (CBN) is a phytocannabinoid with potential therapeutic applications.
- Understanding the molecular interactions of CBN with biological targets like DNA is crucial for its pharmacological evaluation.
- Deoxyribonucleic acid (DNA) can interact with various small molecules, influencing its structure and function.
Purpose of the Study:
- To investigate the interaction mechanism between cannabinol (CBN) and herring sperm DNA.
- To determine the binding affinity and thermodynamic parameters of the CBN-DNA complex.
- To elucidate the role of hydrophobic interactions in the binding process.
Main Methods:
- UV-Vis absorption spectroscopy
- Fluorescence spectroscopy using acridine orange as a probe
- DNA melting temperature analysis
- Molecular docking simulations
- Ionic strength variation studies
Main Results:
- Acridine orange fluorescence quenching by CBN indicates DNA binding.
- Binding constants were determined at three different temperatures.
- Molecular docking results support the experimental spectroscopic findings.
- Hydrophobic interactions were identified as the primary driving force for CBN-DNA binding.
- Ionic strength influences the fluorescence properties of the complex.
Conclusions:
- Cannabinol (CBN) directly interacts with herring sperm DNA.
- The binding is predominantly driven by hydrophobic interactions.
- Spectroscopic and computational methods provide consistent evidence for CBN-DNA complex formation.
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