Related Experiment Video
Updated: Feb 20, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Toward Artificial Mitochondrion: Mimicking Oxidative Phosphorylation in Polymer and Hybrid Membranes
Lado Otrin1, Nika Marušič1, Claudia Bednarz1
1Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.
Abstract:
For energy supply to biomimetic constructs, a complex chemical energy-driven ATP-generating artificial system was built. The system was assembled with bottom-up detergent-mediated reconstitution of an ATP synthase and a terminal oxidase into two types of novel nanocontainers, built from either graft copolymer membranes or from hybrid graft copolymer/lipid membranes. The versatility and biocompatibility of the proposed nanocontainers was demonstrated through convenient system assembly and through high retained activity of both membrane-embedded enzymes. In the future, the nanocontainers might be used as a platform for the functional reconstitution of other complex membrane proteins and could considerably expedite the design of nanoreactors, biosensors, and artificial organelles.
Related Concept Videos
Mitochondrial Membranes
Chemiosmosis
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
The Inner Mitochondrial Membrane
Chemiosmosis and ATP Synthesis
Electron Transport Chains
The ETC is comprised of...
Mitochondria

