Nocardioides immobilis sp. nov., isolated from iron mine soil

A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521^T was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that strain FLL521^T belonged to genus Nocardioides in family Nocardioidaceae, with the highest sequence identity to Nocardioides panacisoli GSoil 346^T (96.3 %). The DNA G+C content of strain FLL521^T was 70.3 mol%. The cell-wall peptidoglycan type was ll-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the predominant fatty acids (>5 %) were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The polar lipids of strain FLL521^T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and three unidentified phospholipids. Strain FLL521^T showed physiological and biochemical differences with the related strains in the Voges-Proskauer test, hydrolysis of l-arginine, utilization of d-mannose, inositol, phenylalanine and acid production from l-arabinose. Based on the phenotypic, genotypic and phylogenetic analyses, strain FLL521^T represents a novel species of Nocardioides, for which the name Nocardioides immobilis sp. nov. is proposed. The type strain is FLL521^T (=KCTC 39931^T=CCTCC AB 2017083^T).