Serum alpha₁ -proteinase inhibitor concentrations in dogs with systemic inflammatory response syndrome or sepsis

To determine whether the concentration of serum canine alpha₁ -proteinase inhibitor (cα₁ -PI) has diagnostic or prognostic utility in dogs with sepsis or noninfectious systemic inflammatory response syndrome (SIRS).

Prospective, observational study from May to December 2010.

University teaching hospital ICU.

Sixty-nine client-owned dogs: 19 dogs with SIRS or sepsis and 50 healthy control dogs.

None.

Serum and plasma samples were collected from dogs with SIRS or sepsis on the day of hospital admission and once on the following 2 days, and on a single day in healthy controls. Patients were assessed using the 10-parameter Acute Patient Physiologic and Laboratory Evaluation (APPLE_full ) and 5-parameter (APPLE_fast ) score. Serum cα₁ -PI concentrations were measured, compared among groups of dogs, and evaluated for a correlation with the concentration of serum C-reactive protein, plasma interleukin-6, tumor necrosis factor-α, the APPLE scores, and survival to discharge. Serum cα₁ -PI concentrations were significantly lower in dogs with SIRS/sepsis (P < 0.001) than in healthy controls. While day 1 serum cα₁ -PI concentrations did not differ between dogs with SIRS and those with sepsis (P = 0.592), septic dogs had significantly lower serum cα₁ -PI concentrations on days 2 (P = 0.017) and 3 (P = 0.036) than dogs with SIRS. Serum cα₁ -PI concentrations did not differ between survivors and nonsurvivors (P = 1.000), but were inversely correlated with the APPLE_full score (ρ = -0.48; P = 0.040) and plasma interleukin-6 concentrations (ρ = -0.50; P = 0.037).

These results suggest a role of cα₁ -PI as a negative acute phase protein in dogs. The concentration of serum cα₁ -PI at the time of hospital admission does not have utility to identify dogs with sepsis from those with noninfectious SIRS, but may be a useful surrogate marker for early stratification of illness severity.