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The Effect of Processing Technique on Fat Graft Survival
Orlando Canizares1,2, Jennifer E Thomson1,2, Robert J Allen1,2
1New York, N.Y.
Plastic and Reconstructive Surgery
|October 26, 2017
Summary
Processing adipose tissue on Telfa gauze enhances fat graft survival. This method increases functional adipocytes and promotes vascularization, leading to improved graft persistence compared to other processing techniques.
Area of Science:
- Regenerative Medicine
- Tissue Engineering
- Adipose-Derived Stem Cells
Background:
- Fat graft survival rates vary significantly.
- Current processing methods may not optimize adipokine profiles for better survival.
Purpose of the Study:
- To investigate the impact of Telfa gauze processing on lipoaspirate and its effect on fat graft survival.
- To determine if Telfa gauze processing enhances functional adipocyte yield and angiogenic factor secretion.
Main Methods:
- Human lipoaspirate was processed using Telfa gauze, centrifugation, or left unprocessed.
- Progenitor cell populations and functional adipocytes were quantified.
- Grafted lipoaspirates in mice were assessed for persistence, vascular density, and growth factor secretion.
Main Results:
- Telfa gauze processing yielded more functional adipocytes and significantly improved fat graft survival (70.9%) after 10 weeks.
- This method also increased vascular density and secretion of vascular endothelial growth factor and platelet-derived growth factor-BB.
Conclusions:
- Lipoaspirate processing significantly impacts fat graft survival.
- Processing adipose tissue on Telfa gauze enhances functional adipocyte numbers and angiogenic factor secretion, improving graft survivability.
