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Published on: March 24, 2015
Mlsa determinants: relationship to Fc gamma receptor and tissue distribution
1Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Abstract:
We have analysed the genetic relationship between Mlsa and Fc gamma R in mice. Using the Fc gamma R-specific DNA probes, we were unable to detect a restriction fragment length polymorphism (RFLP) which is consistent in DNA derived from Mlsa strains and which differed from that of Mlsb strains, while we could see a polymorphism that distinguishes Ly17.1 from Ly17.2, alleles of the Fc gamma R. These results strongly suggest that Mlsa is neither a product of the alpha Fc gamma R nor of the beta Fc gamma R gene. Furthermore, we have re-examined the tissue distribution of Mlsa determinants using a major histocompatibility complex (MHC) class II antigen-positive T-cell tumour as well as a pure population of bone marrow derived macrophages of Mlsa genotype. Both these cell types were recognized to varying degrees by alloreactive cells; however, none of them expressed functionally detectable Mlsa determinants. We conclude from our studies that Mlsa is a highly stimulatory self peptide that is exclusively expressed in B cells.
Insights
The study found that Mlsa is not related to Fc gamma R genes in mice. Mlsa is a highly stimulatory self peptide exclusively expressed in B cells, not on macrophages or T-cell tumors.
Area of Science:
- Immunogenetics
- Molecular Biology
Background:
- The genetic relationship between Mlsa and Fc gamma R in mice remains unclear.
- Fc gamma R (Fc receptor gamma) plays a role in immune responses.
Purpose of the Study:
- To investigate the genetic linkage between Mlsa and Fc gamma R.
- To determine the tissue expression of Mlsa determinants.
Main Methods:
- Analysis of restriction fragment length polymorphism (RFLP) using Fc gamma R-specific DNA probes.
- Examination of Mlsa determinant expression on T-cell tumors and bone marrow-derived macrophages.
Main Results:
- No RFLP was detected between Mlsa and Fc gamma R genes, suggesting no direct genetic relationship.
- Fc gamma R alleles (Ly17.1 and Ly17.2) showed polymorphism.
- Mlsa determinants were not functionally detected on MHC class II antigen-positive T-cell tumors or macrophages, despite recognition by alloreactive cells.
Conclusions:
- Mlsa is not a product of the alpha or beta Fc gamma R genes.
- Mlsa is a highly stimulatory self peptide.
- Mlsa is exclusively expressed in B cells.
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