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CDK1 interacts with iASPP to regulate colorectal cancer cell proliferation through p53 pathway

Wei Gan1, Hua Zhao1, Tiegang Li1

  • 1Department of General Surgery, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, China.

Oncotarget
|October 27, 2017
PubMed

Insights

Cyclin-dependent kinase 1 (CDK1) and iASPP are upregulated in colorectal cancer (CRC) and promote cancer cell proliferation. Their interaction affects CRC cell apoptosis via the p53 pathway, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Cyclin-dependent kinase 1 (CDK1) regulates the G2-M checkpoint and is a potential cancer therapeutic target.
  • Inhibition of Interacting SH3 Domain Protein (iASPP) is crucial for controlling cancer cell proliferation.

Purpose of the Study:

  • To investigate the expression of CDK1 and iASPP in colorectal cancer (CRC).
  • To determine the interaction between CDK1 and iASPP proteins.
  • To elucidate the role of CDK1-iASPP interaction in CRC cell proliferation and apoptosis via the p53 pathway.

Main Methods:

  • Monitoring mRNA and protein expression of CDK1 and iASPP in CRC tissues and cell lines.
  • GST pull-down and Co-immunoprecipitation (Co-IP) assays to confirm protein interaction.
  • Analysis of the p53 apoptosis pathway in CRC cell lines.

Main Results:

  • CDK1 and iASPP were found to be upregulated in CRC tissues and cell lines.
  • Direct interaction between CDK1 and iASPP proteins was confirmed.
  • The CDK1-iASPP interaction was shown to influence CRC cell proliferation and apoptosis, involving the p53 pathway.

Conclusions:

  • CDK1 and iASPP are upregulated in CRC and interact to affect cell proliferation and apoptosis through the p53 pathway.
  • CDK1 and iASPP represent promising therapeutic targets and prognostic markers for CRC.
  • This study provides a novel theoretical basis for targeted CRC therapy based on protein interactions.
Keywords:
CDK1cell proliferationcolorectal canceriASPPp53

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