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Expression of protein kinase C gamma promotes cell migration in colon cancer

Catríona M Dowling1,2, Sheri L Hayes1,2, James J Phelan3

  • 1Graduate Entry Medical School, University of Limerick, Limerick, Ireland.

Oncotarget
|October 27, 2017
PubMed

Insights

Protein Kinase C gamma (PKC gamma) is upregulated in colon cancer, contrary to traditional views. Reducing PKC gamma inhibits cancer cell migration and growth, suggesting its potential as a novel cancer therapy target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein Kinase Cs (PKCs) are implicated in cancer, traditionally viewed as tumor promoters.
  • Emerging evidence suggests PKCs may also function in tumor suppression.
  • Limited patient sample data complicates targeting PKCs in cancer therapy.

Purpose of the Study:

  • To investigate the role of PKC gamma, a largely neglected PKC isozyme, in colon cancer.
  • To analyze PKC gamma expression in patient-matched normal and colon cancer tissues.
  • To elucidate the functional impact of PKC gamma on colon cancer cell behavior.

Main Methods:

  • Gene expression analysis of PKC gamma in 54% of patient colon cancer tissues compared to normal tissues.
  • Functional assays in colon cancer cells to assess the effects of reduced PKC gamma levels on cell migration, foci formation, adhesion, and proliferation.

Main Results:

  • PKC gamma gene expression was significantly upregulated in 54% of colon cancer tissues.
  • Reduced PKC gamma levels in colon cancer cells inhibited cell migration and foci formation.
  • Decreased PKC gamma expression led to increased cell adhesion and proliferation.

Conclusions:

  • PKC gamma plays a critical role in colon cancer progression.
  • PKC gamma represents a potential therapeutic target for colon cancer treatment.
  • Reconsideration of PKC gamma's role in cancer is warranted for developing new therapies.
Keywords:
colon cancerprotein kinase C gammatumor promoter

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