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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
[Levels of T-Lymphocyte Subsets, IL-17, IL-35 and IFN-γ in Peripheral Blood and Their Clinical Significance in
Zhao-Juan Xu1, Dong Zhao2, Fu-Ping Li1
1School of Nursing, Hebei College of Traditional Chinese Medicine, Shijiazhuang 050200, Hebei Province, China.
Objective:
To explore the levels of T-lymphocyte subsets and IL-17, IL-35, IFN-γ in peripheral blood of patients with multiple myeloma(MM) and their clinical significance.
Methods:
A total of 86 MM patients in our hospital from January 2014 to January 2017 were enrolled in MM group and 30 healthy persons were enrolled in control group, the CD4+/CD8+ T cells ratio, CD4+CD25high/+ CD127low/- Treg level in peripheral blood were detected by flow cytometer. The levels of IL-17, IL-35 and IFN-γ in peripheral serum were detected by ELISA, and the differences of detected indicators between different groups were compared.
Results:
Compared with control group, the proportion of CD4+ T cells and CD4+/CD8+ T cells ratio decreased, the proportion of CD8+ T cells and Treg increased in MM group. The differences of T lymphocyte subsets level between group III stage of MM and control group were statistically significant (P<0.05). With enhancing of clinical stages, Treg level showed a increasing trend, especially in III stage (P<0.05), the serum level of IL-17 as followed in turn: III stage>II stage>I stage>control, the serum level of IL-35 and IFN-γ as followed in turn: control>I stage>II stage>III stage (P<0.05). In terms of disease status, the propurtion of Treg cells as fllowed in turn: disease progression stage>stable stage>control (P<0.05), the serum level of IL-17 as followed in turn also: disease progression stage>stable stage (P<0.05), while the serum level of IL-35 and IFN-γ as followed in turn: control>disease table stage>progression stage (P<0.05).
Conclusion:
The abnomal level of T-lymphocyte subsets, Treg, IL-17, IL-35 and IFN-γ are related with progression and prognosis of MM patients.
Insights
Immune system changes, including T-lymphocyte subsets and cytokines like IL-17, IL-35, and IFN-γ, are linked to multiple myeloma progression. These immune markers may indicate disease advancement and patient prognosis.
Area of Science:
- Immunology
- Hematology
- Oncology
Background:
- Multiple myeloma (MM) is a hematologic malignancy characterized by uncontrolled proliferation of plasma cells.
- Immune dysregulation plays a crucial role in the pathogenesis and progression of MM.
Purpose of the Study:
- To investigate the levels of T-lymphocyte subsets (CD4+, CD8+, Treg) and key cytokines (IL-17, IL-35, IFN-γ) in the peripheral blood of MM patients.
- To determine the clinical significance of these immune markers in relation to MM staging and disease status.
Main Methods:
- Flow cytometry was used to quantify CD4+/CD8+ T cell ratios and regulatory T cell (Treg) levels in 86 MM patients and 30 healthy controls.
- Enzyme-linked immunosorbent assay (ELISA) measured serum levels of IL-17, IL-35, and IFN-γ.
- Statistical comparisons were made between different MM stages and disease statuses.
Main Results:
- MM patients exhibited decreased CD4+/CD8+ T cell ratios and increased CD8+ T cells and Tregs compared to controls.
- Treg levels and IL-17 showed a significant increasing trend with advancing MM clinical stages (Stage III) and disease progression.
- Conversely, IL-35 and IFN-γ levels decreased with disease progression, being highest in controls and lowest in Stage III MM.
Conclusions:
- Abnormal levels of T-lymphocyte subsets, Tregs, IL-17, IL-35, and IFN-γ are associated with the progression and prognosis of multiple myeloma.
- These immune markers hold potential as indicators for monitoring MM disease status and predicting patient outcomes.
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