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Updated: Feb 20, 2026
Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel
Published on: June 29, 2015
[Main Factors Influencing FeCl3-Induced Mouse Mesenteric Arteriole Thrombosis Model]
Yang Zhang1, Rong Yan1, Qiong-Ya Cui1
1Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China.
Optimizing the ferric chloride (FeCl3)-induced mouse mesenteric arteriole thrombosis model requires specific conditions. Younger mice (3-6 weeks), higher platelet counts (1x10^8 to 2x10^8), and lower FeCl3 concentrations (6-12%) yield reliable thrombosis.
Area of Science:
- Vascular biology
- Hemostasis and thrombosis
- Animal models of disease
Background:
- The ferric chloride (FeCl3)-induced mouse mesenteric arteriole thrombosis model is a valuable tool for studying thrombotic events.
- Optimizing this model is crucial for reproducible and reliable research outcomes in thrombosis studies.
Purpose of the Study:
- To investigate key factors influencing the FeCl3-induced mouse mesenteric arteriole thrombosis model.
- To determine optimal parameters for establishing a consistent and effective thrombosis model in mice.
Main Methods:
- Platelets were isolated, labeled with Calcein-AM, and transfused into recipient mice.
- The impact of mouse age (3-6, 6-10, >10 weeks), transfused platelet counts (1x10^7, 1x10^8, 2x10^8), and FeCl3 concentrations (6%, 12%, 24%, 48%) on thrombosis were evaluated.
Main Results:
- Younger mice (3-6 weeks) exhibited shorter occlusion times (16 min) compared to older groups.
- Transfusion of 1x10^8 to 2x10^8 platelets resulted in faster occlusion (15-18 min) than 1x10^7 platelets (30 min).
- Lower FeCl3 concentrations (6-12%) led to occlusion times of 15-20 min, while higher concentrations (24-48%) caused rapid occlusion (<10 min).
Conclusions:
- Optimal conditions for FeCl3-induced thrombosis include using 3-6 week-old mice, transfusing 1x10^8 to 2x10^8 labeled platelets, and employing 6-12% FeCl3.
- These optimized parameters facilitate the reliable establishment of the mouse mesenteric arteriole thrombosis model for research.
