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Related Experiment Video

Updated: Feb 20, 2026

Generation of Stable Transgenic C. elegans Using Microinjection
12:09

Generation of Stable Transgenic C. elegans Using Microinjection

Published on: August 15, 2008

54.5K

Caenorhabditis elegans Microinjection.

Matthias Rieckher1, Nektarios Tavernarakis2,3

  • 1Institute for Genome Stability in Aging and Disease, Medical Faculty, Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany.

Bio-Protocol
|October 27, 2017
PubMed
Summary
This summary is machine-generated.

Microinjection is a key method for genetically modifying the nematode Caenorhabditis elegans, enabling gene expression, CRISPR-Cas9 editing, and RNA interference. This protocol details the microinjection process and how to identify transgenic offspring.

Keywords:
Caenorhabditis elegansExtrachromosomal arraysGFPGenetic transformationGermlineMicroinjectionTransgenic animals

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Microinjection is the primary technique for genetic transformation in *Caenorhabditis elegans*.
  • This method allows for the introduction of exogenous DNA into the nematode germline.
  • Applications include transgenic gene expression, genome editing with CRISPR-Cas9, and RNA interference (RNAi) via dsRNA transcription.

Purpose of the Study:

  • To provide a detailed protocol for microinjection in *Caenorhabditis elegans*.
  • To outline the subsequent steps for selecting transgenic progeny.
  • To facilitate reproducible genetic manipulation in this model organism.

Main Methods:

  • Microinjection of exogenous DNA into the oocytes of adult hermaphrodite *C. elegans*.
  • Utilizing the germline for delivery of genetic material.
  • Selection of transgenic offspring from injected animals.

Main Results:

  • Successful delivery of exogenous DNA into *C. elegans* oocytes.
  • Generation of transgenic *C. elegans* among the offspring.
  • Demonstration of the feasibility of gene expression, genome editing, and RNAi.

Conclusions:

  • Microinjection is an effective and widely used method for genetic transformation in *C. elegans*.
  • The described protocol enables the generation and identification of transgenic nematodes.
  • This technique is crucial for advancing research in *C. elegans* genetics and functional genomics.