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Inhibitory Effect of Vaginal Lactobacillus Supernatants on Cervical Cancer Cells
Ke-Di Wang1, Dong-Jiang Xu2, Bao-Ya Wang1
1Department of Clinical Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Probiotics and Antimicrobial Proteins
|October 27, 2017
Summary
Vaginal Lactobacillus supernatants inhibit cervical cancer cell growth by altering cell cycle and human papillomavirus (HPV) oncogene expression. These findings support Lactobacillus
Area of Science:
- Microbiology
- Oncology
- Cell Biology
Background:
- Lactobacilli are known to inhibit cancer cell proliferation.
- The impact of vaginal Lactobacilli on cervical cancer remains underexplored.
Purpose of the Study:
- To investigate the effects of vaginal Lactobacillus supernatants on cervical cancer cell viability.
- To elucidate the underlying mechanisms, including cell cycle regulation and oncogene expression.
Main Methods:
- Cervical cancer (Caski) cells were treated with cell-free supernatants from Lactobacillus crispatus, L. jensenii, and L. gasseri.
- Cell viability was assessed using MTT assays.
- Cell cycle distribution and human papillomavirus (HPV) E6/E7 oncogene expression were analyzed via flow cytometry and RT-PCR.
Main Results:
- Lactobacillus supernatants significantly inhibited Caski cell proliferation in a pH-independent manner.
- Treatment led to an increase in S phase and a decrease in G2/M phase cell populations.
- Expression of HPV E6/E7, CDK2, and cyclin A decreased, while p21 expression increased.
Conclusions:
- Vaginal Lactobacillus supernatants exhibit inhibitory effects on cervical cancer cell viability.
- These effects are mediated through the regulation of HPV oncogenes and cell cycle-related genes.
- Lactobacillus represents a potential therapeutic agent for cervical cancer treatment.
