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Flow Cytometry: The Glass Is Half Full

Howard M Shapiro1

  • 1One World Cytometry, Inc., 283 Highland Ave., West Newton, MA, 02465, USA. hms@shapirolab.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2017
PubMed

Keywords:
Blood cellsCellsCoulter volumeDyesElectrostatic sortingFlow cytometryFluorescenceHemacytometryMicrographiaMicroscopyPoisson statistics

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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