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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Feb 20, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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High Throughput Flow Cytometry for Cell Surface Profiling.

Joshua Paterson1, Laurie E Ailles2

  • 1Princess Margaret Cancer Centre, University Health Network, 610 University Ave., Toronto, ON, Canada, M5G 1L7.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2017
PubMed
Summary

High-throughput flow cytometry enables comprehensive cell surface protein profiling, revealing novel biomarkers and cell populations in complex samples. This cost-effective method enhances biomarker discovery and understanding of cellular heterogeneity.

Keywords:
Antibody arrayBiomarkerCell surface markersHigh throughputMolecular profilingStem cellsSurfaceome

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Area of Science:

  • Cellular and Molecular Biology
  • Immunology
  • Biomarker Discovery

Background:

  • Cell surface proteins are crucial for identifying biomarkers and therapeutic targets.
  • Limited experimental data exists on the surfaceome of individual cells, especially in heterogeneous samples.
  • Transcriptome and surfaceome often show poor correlation, necessitating direct surface protein analysis.

Purpose of the Study:

  • To develop and describe a method for high-throughput cell surface profiling using flow cytometry.
  • To enable the discovery of novel cell surface markers and subpopulations.
  • To provide a cost-effective, scalable approach for analyzing the surfaceome.

Main Methods:

  • Utilized conventional single or multicolor flow cytometry for cell surface protein analysis.
  • Developed a microarray-like screening platform for comprehensive surfaceome interrogation.
  • Adapted the method for flexible antibody panel sizes, enabling customized screening.

Main Results:

  • Demonstrated the capability of flow cytometry to profile cell surface proteins at single-cell resolution.
  • Showcased the platform's ability to reveal unexpected markers and rare cell subpopulations.
  • Generated reliable, cost-effective data from diverse sample types.

Conclusions:

  • High-throughput flow cytometry offers a powerful, scalable solution for cell surface profiling.
  • This approach facilitates the discovery of novel biomarkers and enhances the understanding of cellular heterogeneity.
  • The method supports advanced bioinformatics analyses, such as hierarchical clustering, for deeper insights.