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Related Experiment Video

Updated: Feb 20, 2026

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Immunophenotypic Identification of Early Myeloerythroid Development.

Cornelis J H Pronk1,2, David Bryder3

  • 1Division of Molecular Hematology, Institution for Laboratory Medicine, Lund University, Klinikgatan 26, 221 84, Lund, Sweden. kees-jan.pronk@med.lu.se.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2017
PubMed
Summary

This study details methods for analyzing and purifying immature myeloerythroid cells using multiparameter flow cytometry in mice. These techniques aid in understanding cell development and diseases like leukemia.

Keywords:
Cell isolationDifferentiationErythropoiesis myeloerythropoesisFlow cytometryHematopoiesisImmunophenotypeMyelopoiesis

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Area of Science:

  • Hematology
  • Developmental Biology
  • Immunology

Background:

  • Multipotent hematopoietic stem cells differentiate into various mature blood cell lineages.
  • Understanding early cell development is crucial for insights into diseases like leukemia, which involve blocked differentiation.
  • High-resolution profiling of these developmental stages is essential for mechanistic studies.

Purpose of the Study:

  • To provide protocols for analyzing and purifying immature myeloerythroid lineages.
  • To focus on applications in C57BL/6 mice, facilitating use in genetically modified models.
  • To enable reproducible identification of bone marrow cells using multiparameter flow cytometry.

Main Methods:

  • Multiparameter flow cytometry for cell analysis and purification.
  • Utilizing commercially available reagents for maximal reproducibility.
  • Standard flow cytometer configuration for established protocols.

Main Results:

  • Detailed protocols for the identification and purification of immature myeloerythroid cells.
  • Demonstrated feasibility in C57BL/6 mouse bone marrow.
  • Established methods for reproducible analysis using commercial reagents.

Conclusions:

  • Multiparameter flow cytometry is a powerful tool for studying myeloerythroid cell development.
  • These protocols support research into normal hematopoiesis and leukemogenesis.
  • The methods are suitable for genetically modified mouse models on a C57BL/6 background.