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3D nerve cell cultures and complex physiological relevance.

Xin Cheng¹, Kenneth Ndyabawe¹, Amish Asthana¹

¹School of Chemical, Material, and Biomedical Engineering, College of Engineering, Driftmier Engineering Center, University of Georgia, Athens, GA 30602, USA.

Drug Discovery Today

|October 28, 2017

Summary

This summary is machine-generated.

Complex physiological relevance (CPR) in neuronal cultures is key for drug discovery. This study shows [Ca2+]i oscillation frequency can validate 3D neuronal models, differentiating them from 2D cultures.

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Area of Science:

Tissue engineering
Neuroscience
Drug discovery

Background:

Establishing consensus on complex physiological relevance (CPR) for neuronal cultures remains a challenge in tissue engineering.
CPR of 3D neuronal cultures is crucial for validating in vitro models in drug discovery for neurological diseases and neurotoxicity screening.

Purpose of the Study:

To investigate the potential of intracellular calcium ([Ca2+])i oscillation frequency as a CPR outcome for neuronal cultures.
To demonstrate that [Ca2+])i oscillation frequency can distinguish 3D neuronal cultures from 2D monolayers, indicating in vivo-like behavior.

Main Methods:

Culturing of 2D and 3D neuronal models.
Measurement and analysis of intracellular calcium ([Ca2+])i oscillation frequencies in both culture types.

Main Results:

Intracellular calcium ([Ca2+])i oscillation frequencies were significantly higher in 2D neuronal cultures compared to 3D cultures.
Evidence suggests [Ca2+])i oscillation frequency can serve as a valid indicator of CPR in neuronal models.

Conclusions:

Intracellular calcium ([Ca2+])i oscillation frequency is a promising biomarker for assessing the complex physiological relevance (CPR) of neuronal cultures.
The distinct oscillation frequencies observed between 2D and 3D cultures highlight the superior in vivo-like behavior of 3D models for drug discovery applications.