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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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DNA Isolation01:34

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
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Small-Scale Extraction of Caenorhabditis elegans Genomic DNA
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Efficient isolation method for high-quality genomic DNA from cicada exuviae.

Hoa Quynh Nguyen1, Ye Inn Kim1, Amaël Borzée2

  • 1Department of Life Science Ewha Womans University Seoul Korea.

Ecology and Evolution
|October 28, 2017
PubMed
Summary

Researchers explored DNA extraction from cicada exuviae for genetic studies. The PowerSoil DNA Isolation kit proved most effective, yielding high-quality DNA suitable for sequencing and population genetics research.

Keywords:
Crytptotympana atratacicada exoskeletonexuviaenoninvasive DNA sampling

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Area of Science:

  • Molecular Biology
  • Entomology
  • Conservation Genetics

Background:

  • Ethical concerns drive demand for non-destructive genetic sampling methods.
  • Cicada exuviae (molted skins) are abundant, easily collected, and potential sources of genomic DNA.
  • Previous methods for DNA extraction from exoskeletons have limitations.

Purpose of the Study:

  • To identify the most efficient method for extracting high-quantity, high-quality DNA from cicada exuviae.
  • To compare the efficacy of six different DNA extraction protocols.
  • To assess the suitability of extracted DNA for genetic analyses, including sequencing.

Main Methods:

  • Six DNA extraction protocols (manual and commercial kits) were tested.
  • DNA was extracted from four distinct parts of cicada exuviae.
  • DNA yield, purity, amplification, and sequencing success were evaluated.
  • Spectroscopic measurements were used to assess humic acid contamination.

Main Results:

  • Both extraction protocol and exuvia part significantly impacted DNA yield and purity.
  • Only the PowerSoil DNA Isolation kit yielded DNA of expected size and enabled successful sequencing.
  • Other protocols failed, likely due to low DNA yield and humic acid contamination from soil.

Conclusions:

  • The PowerSoil DNA Isolation kit is recommended for efficient DNA extraction from cicada exuviae.
  • Extracted DNA is suitable for species identification and investigating genetic diversity.
  • This method offers a valuable tool for population genetics and ecological studies.