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Author Spotlight: Advancing Erythropoiesis Research - A Simplified Pipeline for Assessing Hematopoietic Stem Cell Function in Myelodysplastic Syndromes
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Stress Erythropoiesis Model Systems

Laura F Bennett1, Chang Liao1, Robert F Paulson2

  • 1Department of Veterinary and Biomedical Sciences and Center for Molecular Immunology and Infectious Disease. Laura Bennett and Robert Paulson are Intercollege Graduate Program in Genetics. Robert Paulson and Chang Liao are Pathobiology Graduate Program, The Pennsylvania State University, 115 Henning Building, University Park, PA, 16802, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2017
PubMed

Bone marrow steady-state erythropoiesis maintains erythroid homeostasis throughout life. This process constantly generates new erythrocytes to replace the senescent erythrocytes that are removed by macrophages in the spleen. In contrast, anemic or hypoxic stress induces a physiological response designed to increase oxygen delivery to the tissues. Stress erythropoiesis is a key component of this response. It is best understood in mice where it is extramedullary occurring in the adult spleen and liver and in the fetal liver during development. Stress erythropoiesis utilizes progenitor cells and signals that are distinct from bone marrow steady-state erythropoiesis. Because of that observation many genes may play a role in stress erythropoiesis despite having no effect on steady-state erythropoiesis. In this chapter, we will discuss in vivo and in vitro techniques to study stress erythropoiesis in mice and how the in vitro culture system can be extended to study human stress erythropoiesis.

Keywords:
AnemiaBone marrow transplantHypoxiaIn vitro cultureMethylcellulose colony assaysPhenylhydrazine induced acute hemolytic anemiaStress erythropoiesis

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