Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CTCF binding landscape is shaped by the epigenetic state of the N-terminal nucleosome in relation to CTCF motif orientation.

Nucleic acids research·2025
Same author

The <i>Drosophila</i> Estrogen-Related Receptor promotes triglyceride storage within the larval fat body.

bioRxiv : the preprint server for biology·2024
Same author

Generating and testing the efficacy of reagents for CRISPR/Cas9 homology directed repair-based manipulations in Tribolium.

Journal of insect science (Online)·2024
Same author

Glycolytic Disruption Triggers Interorgan Signaling to Nonautonomously Restrict <i>Drosophila</i> Larval Growth.

bioRxiv : the preprint server for biology·2024
Same author

BORIS/CTCFL epigenetically reprograms clustered CTCF binding sites into alternative transcriptional start sites.

Genome biology·2024
Same author

Design and testing of a humanized porcine donor for xenotransplantation.

Nature·2023

Related Experiment Video

Updated: Feb 20, 2026

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
06:59

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Published on: June 26, 2019

12.5K

Discovering a binary CTCF code with a little help from BORIS.

Victor V Lobanenkov1, Gabriel E Zentner2

  • 1a Molecular Pathology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health , 5601 Fishers Ln, Rockville , MD , USA.

Nucleus (Austin, Tex.)
|October 28, 2017
PubMed
Summary
This summary is machine-generated.

CCCTC-binding factor (CTCF) and its paralog BORIS bind DNA similarly but have distinct roles. New research identifies two classes of CTCF target sites, impacting chromatin regulation in germ and cancer cells.

Keywords:
1xCTS2xCTSBORISCTCFCTCFLChIP-seqchromatin

More Related Videos

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
09:40

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Published on: June 27, 2017

8.3K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.4K

Related Experiment Videos

Last Updated: Feb 20, 2026

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
06:59

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Published on: June 26, 2019

12.5K
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
09:40

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Published on: June 27, 2017

8.3K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.4K

Area of Science:

  • Genetics
  • Epigenetics
  • Molecular Biology

Background:

  • CCCTC-binding factor (CTCF) is a key regulator of chromatin architecture.
  • Its paralog, BORIS, shares DNA binding specificity but has divergent termini.
  • CTCF is widely expressed, while BORIS is restricted to germ cells and aberrant in tumors.

Purpose of the Study:

  • To investigate the regulation of genomic co-occupancy by CTCF and BORIS.
  • To identify distinct classes of CTCF target sequences and their functional implications.

Main Methods:

  • Analysis of CTCF and BORIS binding sites in genomic DNA.
  • Characterization of chromatin structural and functional features of different CTCF target site classes.

Main Results:

  • Discovery of two major classes of CTCF target sequences: single (1xCTS) and double (2xCTS) CTCF motifs.
  • Demonstration that 2xCTSes possess distinct functional and chromatin structural features compared to 1xCTS regions.
  • Evidence suggesting differential roles for these classes in chromatin regulation.

Conclusions:

  • The distinct classes of CTCF binding regions (1xCTS and 2xCTS) likely play different roles in chromatin regulation.
  • These findings may advance understanding of heritable epigenetic regulation in normal germ cells and cancer cells.
  • Further research into CTCF and BORIS co-occupancy is warranted.