Modification of porous PLGA microspheres by poly-l-lysine for use as tissue engineering scaffolds

Due to their good biocompatibility, biodegradability and special shapes, porous poly(lactic-co-glycolic acid) (PLGA) microspheres show a wide application in the field of tissue engineering. Herein we demonstrate a simple and low-cost method for modifying porous PLGA microspheres with poly-l-lysine (PLL) to promote cell growth on the microspheres. Porous PLGA microspheres were first treated by sodium hydroxide (NaOH) solution to introduce carboxyl groups on their surface. Then, the hydrolyzed microspheres (PLGA-H) were immerged in PLL solution to yield PLL-impregnated microspheres (PLGA-PLL). Cell experiments showed that although the cytotoxicity of microspheres was slightly increased after PLL modification, their cell viability was still higher than 85%. Compared with PLGA and PLGA-H microspheres, PLGA-PLL microspheres were more favorable for MG63 cell to attach and proliferate due to their increased initial cell attachment numbers and enhanced cell-matrix interactions. This new modification method of porous PLGA microspheres proposes a route toward efficient repair of tissue defects at reduced risk and cost level.