Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intersectin associates with synapsin and regulates its nanoscale localization and function.

Fabian Gerth1, Maria Jäpel2, Arndt Pechstein1,2

  • 1Faculty of Biology, Chemistry, and Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|October 29, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of a STRAIGHT-IN Dual AAVS1 hiPSC line with orthogonal landing pads for versatile DNA payload integration.

Stem cell research·2026
Same author

Generation of four human induced pluripotent stem cell lines derived from patients with corticosteroid-associated central serous chorioretinopathy.

Stem cell research·2026
Same author

<i>ATG</i>8 and protein ATG8ylation - more than just <i>A</i>nother <i>T</i>a<i>G</i>?

Autophagy·2026
Same author

Condensate-mediated shape transformations of cellular membranes by capillary forces.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis.

Molecular metabolism·2026
Same author

Expression and Functional Characterization of Recombinant HLA-DM.

Methods in molecular biology (Clifton, N.J.)·2026

Intersectin protein regulates synaptic vesicle replenishment by linking the reserve pool to the endocytic machinery. This finding is crucial for understanding sustained neurotransmission during high brain activity.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Neurotransmission relies on synaptic vesicle (SV) release and replenishment.
  • Synapsins maintain the reserve pool of SVs for sustained neurotransmission.

Purpose of the Study:

  • To identify regulators of SV replenishment cooperating with synapsins.
  • To elucidate the role of intersectin in SV replenishment.

Main Methods:

  • Biochemical assays to detect protein-protein interactions.
  • Genetic manipulation of intersectin in mice.
  • Analysis of SV distribution and neurotransmission.

Main Results:

  • Intersectin directly binds to synapsin I.
Keywords:
NMR spectroscopyintramolecular regulationmultidomain scaffoldneurotransmissionsynaptic vesicles

Related Experiment Videos

  • Deletion of intersectin impairs SV replenishment and sustained neurotransmission.
  • Wild-type intersectin rescues these defects.

    • Conclusions:

    • Intersectin acts as an autoinhibited scaffold linking the synapsin-dependent SV reserve pool to presynaptic endocytosis.
    • Intersectin is essential for maintaining SV replenishment and sustained neurotransmission.