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Related Experiment Videos

RElative QUantitation Inferred by Evaluating Mixtures (REQUIEM).

Sami T Tuomivaara1, Paul Schliekelman2, Alison V Nairn3

  • 1Complex Carbohydrate Research Center, 315 Riverbend Rd, Athens, GA 30602, USA.

Analytica Chimica Acta
|October 29, 2017
PubMed
Summary

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We developed REQUIEM, a novel method for accurate relative quantitation. This label-free approach enhances data accuracy by diagnosing and eliminating errors in analytical workflows.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Data Science

Background:

  • Accurate relative quantitation is crucial in various scientific fields.
  • Existing strategies for assessing data accuracy are often limited in applicability and ease of implementation.
  • Challenges persist in reliably quantifying analyte levels across different samples.

Purpose of the Study:

  • To introduce REQUIEM, a novel label-free approach for improving and assessing data accuracy in relative quantitation.
  • To provide a generally applicable strategy for unbiased analyte fold-change determination.
  • To enable the diagnosis and elimination of various types of errors in analytical data.

Main Methods:

  • Development of the REQUIEM algorithm for processing analytical data.
Keywords:
AccuracyMass spectrometryPrecisionRNA-SeqRelative quantitation

Related Experiment Videos

  • Preparation of sample mixtures from aliquots of original samples for parallel analysis.
  • Application of the REQUIEM approach to tandem mass spectrometry using isomeric oligosaccharides.

    • Main Results:

    • The REQUIEM algorithm provides unbiased analyte fold-changes and associated statistics.
    • Extensive simulations and standard sample analyses confirm the rigorous foundation of REQUIEM.
    • The method successfully identified inaccuracies in real-world analytical techniques.

    Conclusions:

    • REQUIEM offers a robust and implementable solution for enhancing data accuracy in relative quantitation.
    • The approach effectively diagnoses and eliminates errors often missed by traditional methods.
    • REQUIEM is applicable across diverse analytical techniques, including mass spectrometry.