Related Experiment Video
Updated: Aug 10, 2026

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
Published on: July 27, 2022
Bleaching of a Discolored Tooth with Retrieval of Remnants after Successful Regenerative Endodontics
Aovana Timmerman1, Peter Parashos1
1Melbourne Dental School, University of Melbourne, Melbourne, Victoria, Australia.
Introduction:
This report presents the retrieval of remnants from a discolored mandibular right second premolar (tooth #29) of a 17-year-old female after a successful regenerative endodontic procedure (REP).
Methods:
The REP was performed in October 2011. Coronal discoloration became of great concern to the patient at a review visit in 2016. A cone-beam computed tomographic scan was taken to investigate the formation of hard tissues within the root canal as well as a region of no calcified tissue formation. During internal bleaching of tooth #29, a black material with particulate inclusions was retrieved and examined histopathologically.
Results:
Five years after the REP, there was complete periapical healing, hard tissue formation within the root canal, and complete maturation of the root apex. A hard tissue bridge was noted at the cementoenamel junction when the pulp chamber of #29 was reaccessed. The remnants retrieved from the pulp chamber were confirmed to be partly mineral trioxide aggregate (MTA). A normal tooth color was achieved after 3 weeks of internal bleaching.
Conclusions:
MTA remnants within the pulp chamber contributed to the tooth discoloration and appear to have obstructed hard tissue formation. A matrix of oxidized regenerated cellulose seems not to hinder tissue regeneration and is resorbed by these tissues. Other materials with color stability should be selected as coronal barriers for REPs to avoid a potential adverse effect of the MTA on the REP outcome and discoloration.

