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Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA

Ying Wu1,2, Qian Xiong1, Siting Li1,2

  • 1From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Molecular & Cellular Proteomics : MCP
|October 29, 2017
PubMed

Insights

Long noncoding RNA HOTAIR influences hepatocellular carcinoma (HCC) progression. This study reveals HOTAIR regulates opioid growth factor receptor (OGFr) expression, impacting cell proliferation in HCC.

Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Long noncoding RNA HOTAIR is implicated in human cancers, including hepatocellular carcinoma (HCC).
  • The precise molecular mechanisms of HOTAIR in HCC remain largely unexplored.

Purpose of the Study:

  • To systematically investigate the regulatory functions of HOTAIR in HCC using integrated transcriptomic and proteomic analyses.
  • To elucidate the molecular pathways influenced by HOTAIR in HCC development.

Main Methods:

  • Integrated transcriptomic and quantitative proteomic analysis following HOTAIR inhibition.
  • Bioinformatic analysis of differentially expressed genes (DEGs) and proteins (DEPs).
  • Validation using quantitative RT-PCR, Western blot, and parallel reaction monitoring (PRM).

Main Results:

  • HOTAIR inhibition led to significant dysregulation of 673 transcripts and 293 proteins.
  • DEGs and DEPs were enriched in cancer-related signaling pathways.
  • HOTAIR regulates opioid growth factor receptor (OGFr) expression, affecting HCC cell proliferation.

Conclusions:

  • This study provides novel insights into HOTAIR's functional mechanisms in HCC.
  • HOTAIR's role in HCC cell proliferation is, in part, mediated by OGFr regulation.
  • Integrated omics data and functional studies advance understanding of HOTAIR in HCC.

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