Related Experiment Video
Updated: Feb 19, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
Published on: June 20, 2018
Sequencing and comparative analysis of the Amblyomma sculptum mitogenome
Paulo Henrique Costa de Lima1, Rafael Mazioli Barcelos2, Raphael Contelli Klein3
1Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil; Federal Institute of Education, Science and Technology of Amazonas, Amazonas, Brazil.
Abstract:
The mitogenome of Amblyomma sculptum was sequenced, providing important information for understanding the evolutionary relationships among species of the A. cajennense complex. The mitochondrial genome has a circular structure with 37 genes, including 13 coding DNA sequences, two ribosomal RNA genes (12S rRNA and 16S rRNA) and 22 tRNA genes. Comparative analysis with the mitogenomes of six reference species of the genus Amblyomma revealed that the ND5 gene, which is related to energy metabolism, and control regions 1 and 2 of the mitogenomes have polymorphisms that can be exploited as molecular markers to differentiate A. sculptum from other tick species in the Amblyomma cajennense complex as well as other Amblyomma species.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Genome Annotation and Assembly

