Multiphoton microscopy: A novel diagnostic method for solid tumors in a prospective pediatric oncologic cohort, an
Oliver J Muensterer1, Sibylle Waldron1, Yoon Jung Boo2
1Department of Pediatric Surgery, University Medicine Mainz, Germany.
International Journal of Surgery (London, England)
|October 31, 2017
Summary
Multiphoton microscopy (MPM) accurately diagnoses pediatric solid tumors, comparable to conventional methods. This real-time imaging technique offers potential for intraoperative diagnostics without tissue processing.
Area of Science:
- Oncology
- Pathology
- Medical Imaging
Background:
- Accurate staging and local control are crucial for pediatric solid tumor prognosis.
- Current surgical margin assessment relies on macroscopic evaluation and intraoperative cryosection.
- Multiphoton microscopy (MPM) offers real-time tissue imaging without extensive sample preparation.
Purpose of the Study:
- To evaluate the diagnostic capabilities of MPM for pediatric solid tumors.
- To compare MPM's diagnostic performance against routine histopathology.
Main Methods:
- Pediatric tumor samples (nephroblastoma, neuroblastoma, ganglioneuroma, pleuropulmonary blastoma, hepatocellular carcinoma) were analyzed.
- MPM imaging was directly compared with conventional light microscopy on prepared slides.
- MPM was applied to native tumor tissue to assess visualization through the tumor capsule.
Main Results:
- MPM accurately diagnosed all 7 analyzed pediatric solid tumors.
- The technique visualized tumors through the capsule without sectioning or staining.
- MPM image quality was sufficient for diagnosis and identifying architectural changes.
Conclusions:
- MPM is a viable alternative to conventional histopathology for diagnosing pediatric solid tumors.
- MPM provides real-time intraoperative diagnostic potential, eliminating the need for fixation and staining.
- This technique shows promise as a replacement for frozen section analysis.
