Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

905
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
905

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of Fecal Elastase-1 and Nutritional Status in Dementia.

Clinical nutrition ESPEN·2026
Same author

Ramadan fasting and circadian ambulatory blood pressure patterns in adults aged ≥ 80 years.

BMC geriatrics·2026
Same author

Response to commentary on "Association of four or more rib fractures with complications in isolated blunt chest trauma".

The American journal of emergency medicine·2026
Same author

Combined Excisional Biopsy and Reconstruction With Eccentric Anterior Lamellar Keratoplasty in the Treatment of Corneal Limbal Dermoid.

Cornea·2026
Same author

Reply to Editor by Drs. Lam and Au regarding our article, "Treatment of Choroidal Metastasis with Combined Photodynamic Therapy and Bevacizumab and Comparison with Photodynamic Therapy Alone."1.

Retina (Philadelphia, Pa.)·2026
Same author

Response to the letter to the editor.

Patient education and counseling·2026

Related Experiment Video

Updated: Feb 19, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

6.1K

Human iris three-dimensional imaging at micron resolution by a micro-plenoptic camera.

Hao Chen1, Maria A Woodward2, David T Burke3

  • 1Department of Mechanical Engineering, University of Michigan, 1231 Beal Avenue, Ann Arbor, MI 48109, USA.

Biomedical Optics Express
|October 31, 2017
PubMed
Summary

A novel micro-plenoptic system captures detailed 3D iris topography with high accuracy. This imaging technique shows potential for diagnosing and monitoring iris conditions like naevi and melanoma.

Keywords:
(110.6880) Three-dimensional image acquisition(170.3880) Medical and biological imaging(170.3890) Medical optics instrumentation(330.4460) Ophthalmic optics and devices

More Related Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.0K
Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

1.3K

Related Experiment Videos

Last Updated: Feb 19, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

6.1K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.0K
Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

1.3K

Area of Science:

  • Biomedical optics
  • Ophthalmic imaging
  • Medical diagnostics

Background:

  • Accurate 3D imaging of the anterior iris surface is crucial for diagnosing and monitoring ocular conditions.
  • Existing methods may lack the resolution, speed, or simplicity required for routine clinical use.

Purpose of the Study:

  • To design and validate a micro-plenoptic imaging system for single-shot, high-resolution 3D anterior iris topography.
  • To assess the system's accuracy, precision, and capability in imaging normal and pathological irides.

Main Methods:

  • Development of a micro-plenoptic system for capturing 3D iris surface topography.
  • Single-shot imaging within a 2.4 mm depth-of-field.
  • Evaluation of depth resolution, accuracy, and precision (<20% error).

Main Results:

  • Achieved 10 µm depth resolution with <20% accuracy and precision.
  • Successfully imaged healthy iris structures (ridges and folds, 10-80 µm height differences).
  • Quantified iris melanoma elevation (50 ± 10 µm) and flat surface of iris naevi.

Conclusions:

  • The micro-plenoptic system provides accurate and precise 3D iris topography.
  • Demonstrated potential for clinical applications in iris disease diagnosis.
  • Offers a simple, single-shot method for continuous iris monitoring.