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Related Experiment Video

Updated: Feb 19, 2026

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

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Murine Bronchoalveolar Lavage.

Fan Sun1,2, Gutian Xiao1,2, Zhaoxia Qu1,2

  • 1University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, USA.

Bio-Protocol
|October 31, 2017
PubMed
Summary
This summary is machine-generated.

Bronchoalveolar lavage (BAL) in mice yields cells and fluids for analyzing immune cells, pathogens, and gene expression. This technique aids in understanding lung disease mechanisms and developing targeted therapies.

Keywords:
Bronchoalveolar lavageHema3-stainingLung cancer

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Area of Science:

  • Pulmonology
  • Immunology
  • Cell Biology

Background:

  • Bronchoalveolar lavage (BAL) is a crucial technique for respiratory research.
  • Analyzing BAL samples provides insights into lung physiology and pathology.

Purpose of the Study:

  • To describe a basic Bronchoalveolar lavage (BAL) procedure in mice.
  • To outline the analytical methods applicable to BAL samples for lung disease research.

Main Methods:

  • Bronchoalveolar lavage (BAL) procedure in mice.
  • Analysis of BAL cells and fluids using Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, ELISA, and Luminex assays.

Main Results:

  • BAL enables examination of immune cells, pathogens, and protein levels (cytokines/chemokines).
  • Gene expression analysis of BAL cells can identify inflammation-related markers.

Conclusions:

  • BAL procedures in mice are fundamental for investigating lung disease mechanisms.
  • Comprehensive analysis of BAL samples supports the development of targeted therapeutic strategies for lung diseases.