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The nuclear DEK interactome supports multi-functionality.

Eric A Smith1, Eric F Krumpelbeck1, Anil G Jegga2

  • 1Department of Oncology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, 45219.

Proteins
|October 31, 2017
PubMed
Summary
This summary is machine-generated.

Researchers identified new DEK oncoprotein interactions, revealing its roles in nucleotide synthesis and ribosome formation. This study enhances understanding of DEK

Keywords:
DDX21DEKIMPDH2RPAinteractomemass spectrometryribosome

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Area of Science:

  • Oncology
  • Molecular Biology
  • Proteomics

Background:

  • DEK oncoprotein is overexpressed in various cancers.
  • Known DEK functions involve chromatin structure, epigenetics, and transcription.
  • Previous studies focused on DEK's DNA-binding and chromatin processing.

Purpose of the Study:

  • To develop the first DEK interactome model.
  • To identify novel DEK binding partners and functions in underexplored areas.
  • To generate mechanism-driven hypotheses for DEK activities.

Main Methods:

  • Utilized tandem-affinity purification coupled with mass spectrometry.
  • Investigated protein-protein interactions of the DEK oncoprotein.

Main Results:

  • Identified IMPDH2, DDX21, and RPL7a as novel DEK binding partners.
  • Discovered a hydroxyurea-specific interaction between DEK and replication protein A (RPA).
  • These findings suggest new roles for DEK in nucleotide biosynthesis and ribosome formation, and in response to DNA replication stress.

Conclusions:

  • The DEK interactome model reveals diverse cellular pathway involvement for DEK.
  • DEK plays roles in de novo nucleotide biosynthesis and ribosome formation.
  • A DEK-RPA complex may form during DNA replication fork stalling.