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[Genetic manipulation in zebrafish].

Yu Gao1, Jiahui Liu1, Xin Wang1

  • 1MOE Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 31, 2017
PubMed
Summary
This summary is machine-generated.

Zebrafish are a key model organism for studying development and disease due to advanced genetic tools. This overview covers methods like mutagenesis, gene knockdown, and genome editing for functional genomics.

Keywords:
gene editinggenetic manipulationmutationtransgenictransposonzebrafish

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Area of Science:

  • Developmental Biology
  • Genomics
  • Model Organisms

Background:

  • Zebrafish are increasingly utilized in developmental biology and human disease research.
  • High-resolution live imaging and genetic manipulation techniques enhance zebrafish utility.
  • Zebrafish offer advantages for rapid, cost-effective functional genomics in vertebrates.

Purpose of the Study:

  • To provide a comprehensive overview of genetic manipulation techniques in zebrafish.
  • To highlight the principles behind various genetic modification strategies.
  • To showcase zebrafish as a powerful model for gene function studies.

Main Methods:

  • Ethylnitrosourea (ENU) and insertional mutagenesis
  • Gene trapping and Morpholino-mediated gene knockdown
  • Targeting Induced Local Lesions In Genomes (TILLING)
  • Genome editing using engineered nucleases: Zinc Finger Nucleases (ZFN), Transcription Activator-Like Effector Nucleases (TALEN), and CRISPR/Cas9 system
  • Transgenic methods

Main Results:

  • Detailed explanation of diverse genetic manipulation principles in zebrafish.
  • Coverage of both classical and cutting-edge genome editing technologies.
  • Demonstration of the versatility of zebrafish for functional genomics.

Conclusions:

  • Zebrafish genetic manipulation tools are crucial for advancing developmental biology and disease modeling.
  • The described methods facilitate efficient functional characterization of genes.
  • Zebrafish remain a premier model organism for vertebrate functional genomics.