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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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What is Genetic Engineering?00:49

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Overview
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Related Experiment Video

Updated: Feb 19, 2026

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

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[Recent progresses in CRISPR genome editing in plants].

Hong Li1, Kabin Xie1

  • 1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 31, 2017
PubMed
Summary

CRISPR/Cas9 genome editing revolutionizes plant science, enabling rapid, low-cost gene editing for crop improvement. New CRISPR technologies enhance precision and efficiency for advanced genetic manipulation.

Keywords:
CRISPR/Cas9cropgene targetinggenetic improvementprogress

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Area of Science:

  • Plant Sciences
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 has emerged as a revolutionary tool in life sciences over the past four years.
  • It offers unprecedented throughput, scalability, speed, and cost-effectiveness for editing plant genes.

Purpose of the Study:

  • To review the advancements and applications of CRISPR/Cas9 technology in plant genome editing.
  • To highlight the potential of CRISPR technology in accelerating crop genetic improvement.

Main Methods:

  • Review of CRISPR/Cas9 applications including gene knock-in/knock-out, activation, and suppression.
  • Discussion of advancements in accuracy, capacity, and efficiency of genome editing.
  • Inclusion of emerging technologies like CRISPR/Cpf1 and single base editing.

Main Results:

  • CRISPR/Cas9 enables sophisticated genetic manipulation with improved accuracy and efficiency.
  • The technology facilitates targeted gene activation and suppression in plants.
  • New CRISPR-based tools expand the genome editing toolbox for complex genetic modifications.

Conclusions:

  • CRISPR technology is nearing its potential as an ideal tool for plant sciences.
  • Precise genome editing using CRISPR will significantly accelerate crop genetic improvement.