Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of CD4 and CD2 receptor function.

S J Goldman1, B E Bierer, S J Burakoff

  • 1Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.

Princess Takamatsu Symposia
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ethical Challenges in Clinical Research During the COVID-19 Pandemic.

Journal of bioethical inquiry·2020
Same author

Activation-induced cellular accumulation of histamine in immature but not mature murine mast cells.

Cellular and molecular life sciences : CMLS·2008
Same author

Interleukin-11.

BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy·2007
Same author

Suicide, substances, and stories: treating a teenager in trouble.

Harvard review of psychiatry·2001
Same author

T cell regulation of p62(dok) (Dok1) association with Crk-L.

The Journal of biological chemistry·2001
Same author

A multicenter, randomized, double-blind comparison of different doses of intravenous immunoglobulin for prevention of graft-versus-host disease and infection after allogeneic bone marrow transplantation.

Bone marrow transplantation·2001
Same journal

Survival after liver transplantation in patients with hepatocellular carcinoma.

Princess Takamatsu symposia·1995
Same journal

Surgical treatment of hepatocellular carcinoma.

Princess Takamatsu symposia·1995
Same journal

Treatment of unresectable hepatocellular carcinoma: targeted therapies using iodized oil.

Princess Takamatsu symposia·1995
Same journal

The present status of treatments for hepatocellular carcinoma.

Princess Takamatsu symposia·1995
Same journal

Prospects for prophylactic and therapeutic hepatitis C virus vaccines.

Princess Takamatsu symposia·1995
Same journal

Association of cytotoxic T lymphocyte (CTL) escape mutations with persistent hepatitis C virus (HCV) infection.

Princess Takamatsu symposia·1995
See all related articles

This study reveals key regions of CD4 and CD2 molecules crucial for T cell activation. Specific antibody binding to CD4 and CD2/LFA-3 interactions enhance T cell responses, offering insights into immune cell signaling.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Signaling

Background:

  • The CD4 and CD2 surface receptors play critical roles in T cell activation.
  • Understanding the specific functional domains of these receptors is essential for deciphering T cell-mediated immune responses.

Purpose of the Study:

  • To investigate the functional significance of CD4 and CD2 molecules in T cell activation using a murine hybridoma model.
  • To identify specific regions within CD4 and CD2 that are critical for ligand binding and downstream signaling.

Main Methods:

  • Expression of human CD4 and CD2 in a murine hybridoma (155.16) responsive to HLA-DR.
  • Utilized monoclonal antibodies (mAbs) against CD4 and CD2 to probe functional regions.
  • Assessed interleukin-2 (IL-2) production and intracellular calcium mobilization [(Ca2+]i) as readouts of T cell activation.

Related Experiment Videos

  • Analyzed wild-type and mutated CD2 molecules to determine the role of specific domains in LFA-3 binding and T cell activation.

    • Main Results:

    • CD4+ and CD2+ hybridomas showed enhanced IL-2 production upon stimulation, confirming receptor functionality.
    • Two distinct regions of the CD4 molecule were identified: one (Leu3a binding site) enhancing, and another (OKT4F binding site) inhibiting CD4-dependent functions.
    • Two regions in the external domain of CD2 were found to be involved in Lymphocyte-Function Associated Antigen-3 (LFA-3) binding.
    • CD2/LFA-3 interaction enhanced T cell activation in a manner dependent on the CD2 cytoplasmic domain.

    Conclusions:

    • Specific regions of CD4 and CD2 molecules are critical for mediating T cell activation.
    • The CD2/LFA-3 interaction acts as a stimulatory signal in T cell activation, highlighting its importance in immune responses.