Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

2.9K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
2.9K
Internal Receptors01:31

Internal Receptors

74.9K
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.9K
Types of Receptors: Internal Receptors01:07

Types of Receptors: Internal Receptors

32.9K
Many cellular signals are hydrophilic and cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind intracellular receptors that reside within the cell cytoplasm or nucleus. Many mammalian steroid hormones and nitric oxide (NO) gas use this cell signaling mechanism.
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
32.9K
Intracellular Hormone Receptors01:08

Intracellular Hormone Receptors

60.0K
Lipid-soluble hormones diffuse across the plasma and nuclear membrane of target cells to bind to their specific intracellular receptors. These receptors act as transcription factors that regulate gene expression and protein synthesis in the target cell
60.0K
Drug-Receptor Bonds01:25

Drug-Receptor Bonds

4.9K
Drug-receptor bonds are formed through various chemical forces when drugs interact with target cells. Covalent bonds, strong and irreversible, are exemplified by DNA-alkylating anticancer agents that inhibit cell division. However, such irreversible drug binding lacks selectivity and can modify the DNA of the surrounding healthy cells. Covalent binding often contributes to tissue toxicity, as seen with chloroform and paracetamol metabolites binding to the liver, causing hepatotoxicity.
In...
4.9K
Drug-Receptor Interactions01:29

Drug-Receptor Interactions

7.7K
Drug-receptor interaction describes the binding of receptors by drugs, but not all drug-receptor interactions result in activation and tissue response. For instance, the binding of agonists activates the receptor to generate a cellular reaction, while antagonists bind to receptors without causing their activation.
Several parameters, such as the drug's affinity for its receptor and its efficacy, which is its ability to activate the receptor, determine the drug's effect on the tissue....
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triple collaborative consistency with Mamba for semi-supervised 3D medical image segmentation.

Medical physics·2026
Same author

Dual-Channel Interdigitated Aptamer-Based Sensors for Rapid Small-Molecule Detection in Biofluids.

Angewandte Chemie (International ed. in English)·2026
Same author

Cargo-defined engineered vesicles enable targeted miRNA delivery for cardiac repair after myocardial infarction.

Biomaterials·2026
Same author

Structural modification of 5-alkyl-4-phenyl-thiazole-based fatty acid amide hydrolase and soluble epoxide hydrolase inhibitors to alleviate orofacial inflammatory pain in the rat.

BBA advances·2026
Same author

Performance of immobilized denitrifying bacteria-enhanced ecological floating island for treating actual nitrogenous wastewater.

Frontiers in microbiology·2026
Same author

Sphingosine-1-phosphate - sphinganine-1-phosphate Imbalance Drives Airway Hyperreactivity.

medRxiv : the preprint server for health sciences·2026

Related Experiment Video

Updated: Feb 19, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

13.2K

High-Affinity Nucleic-Acid-Based Receptors for Steroids.

Kyung-Ae Yang, Hyosun Chun1, Yameng Zhang

  • 1School of Computer Science and Engineering, Seoul National University , Seoul 08826, Korea.

ACS Chemical Biology
|October 31, 2017
PubMed
Summary

Researchers developed novel deoxyribonucleotide-based receptors with high affinity for nonaromatic steroids. These aptamers show promise for clinical chemistry applications due to their specific binding capabilities.

More Related Videos

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
11:07

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

Published on: September 21, 2011

16.9K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.4K

Related Experiment Videos

Last Updated: Feb 19, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

13.2K
Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
11:07

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

Published on: September 21, 2011

16.9K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Artificial receptors often exhibit limited affinity and selectivity for hydrophobic molecules.
  • Deoxyribonucleotides offer a versatile scaffold for designing molecular recognition elements.

Purpose of the Study:

  • To develop high-affinity, selective deoxyribonucleotide-based receptors for nonaromatic steroids.
  • To explore the diversity of nucleic acid binding motifs for hydrophobic targets.

Main Methods:

  • Utilized high-stringency selection and tailored counter-selection techniques.
  • Investigated receptor-steroid interactions using analog studies.

Main Results:

  • Identified three new classes of high-affinity deoxyribonucleotide receptors for nonaromatic steroids.
  • Two classes preferentially bind non-hydroxylated steroids, while a third accommodates hydroxyl groups.
  • Demonstrated the potential for aptamers to inhibit double-helix formation.

Conclusions:

  • The study highlights the broad potential of nucleic acids in creating diverse hydrophobic binding motifs.
  • These novel aptamers offer significant promise for advancing clinical chemistry applications through precise molecular recognition.