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Upregulation of the ALDOA/DNA-PK/p53 pathway by dietary restriction suppresses tumor growth

D Ma1,2, X Chen1,2, P-Y Zhang1,2

  • 1Digestive Cancer Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.

Oncogene
|October 31, 2017
PubMed

Insights

Dietary restriction (DR) suppresses tumor growth by activating the aldolase A (ALDOA)/DNA-dependent protein kinase (DNA-PK)/p53 pathway. This novel mechanism highlights how DR enhances the tumor suppressor p53

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Dietary restriction (DR) is known to delay tumor incidence and growth, but the underlying molecular mechanisms remain unclear.
  • Understanding how DR exerts its antitumor effects is crucial for developing novel cancer therapies.

Purpose of the Study:

  • To identify the novel signaling pathway responsible for the antitumor effects of dietary restriction.
  • To elucidate the role of aldolase A (ALDOA) in mediating DR-induced tumor suppression.

Main Methods:

  • Xenograft tumor models were used to study the effects of DR on tumor growth.
  • Immunoprecipitation and Western blotting were employed to investigate protein interactions and activation.
  • Analysis of aldolase A (ALDOA), DNA-dependent protein kinase (DNA-PK), and p53 expression and activity was performed.

Main Results:

  • Dietary restriction upregulated aldolase A (ALDOA) expression in xenograft tumors.
  • ALDOA was found to interact with and activate DNA-dependent protein kinase (DNA-PK).
  • Activated DNA-PK phosphorylated and enhanced the activity of the tumor suppressor p53, leading to suppressed tumor growth. DR's antitumor effects were dependent on p53 presence.

Conclusions:

  • The ALDOA/DNA-PK/p53 signaling pathway is a key mechanism through which dietary restriction exerts its antitumor effects.
  • Aldolase A (ALDOA) acts as a crucial mediator, linking dietary restriction to the activation of the p53 tumor suppressor pathway.
  • Targeting the ALDOA/DNA-PK/p53 axis may represent a promising therapeutic strategy for enhancing the benefits of dietary restriction in cancer treatment.

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