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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

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Related Experiment Video

Updated: Feb 19, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

The multidimensional mechanisms of long noncoding RNA function.

Francesco P Marchese^1,2, Ivan Raimondi^1,2, Maite Huarte^3,4

¹University of Navarra, Center for Applied Medical Research (CIMA), Pamplona, 31008, Spain.

|November 1, 2017

Summary

This summary is machine-generated.

Long noncoding RNAs (lncRNAs) are abundant transcripts regulating gene expression. This review explores emerging mechanisms of lncRNA function within the cell nucleus, highlighting their interactions and transcriptional regulation.

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Last Updated: Feb 19, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

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Published on: July 22, 2016

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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Published on: April 10, 2018

Chromatin Isolation by RNA Purification ChIRP

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Published on: March 25, 2012

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

Recent discoveries reveal that most cellular transcripts are non-protein-coding, including numerous long noncoding RNAs (lncRNAs).
Emerging evidence indicates lncRNAs play significant roles in regulating gene expression through various molecular mechanisms.

Purpose of the Study:

To review and synthesize current understanding of the mechanistic roles of lncRNAs in gene regulation within the cell nucleus.
To discuss the functional interactions of lncRNAs with other cellular molecules and the link between their transcription and biological function.

Main Methods:

Literature review of recent studies on lncRNA mechanisms.
Analysis of functional interactions and transcriptional regulation of lncRNAs.
Comparative discussion of lncRNA-specific versus shared regulatory mechanisms.

Main Results:

lncRNAs employ diverse mechanisms to regulate gene expression in the nucleus.
Functional interactions with proteins, DNA, and other RNAs are key to lncRNA function.
The transcription of lncRNAs is intrinsically linked to their regulatory roles.

Conclusions:

lncRNAs represent a major class of regulatory molecules in the genome.
Understanding lncRNA mechanisms provides new insights into gene regulation.
Further research into lncRNA-specific and shared regulatory pathways is warranted.