Antigenic and immunogenic evaluation of Helicobacter pylori FlaA epitopes
Mansoor Zarei1, Ghasem Mosayebi2, Behzad Khansarinejad2
1Department of Medical Biotechnology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Objectives:
Helicobacter pylori are among most common human pathogens affecting at least half of the world's population. Mobility is one of the important primary factors in bacterial colonization and invasion. The purpose of this research is cloning, expression, and purification of FlaA protein specific epitopes in order to evaluate their antigenicity and immunogenicity.
Materials And Methods:
The antigenic region of the flaA gene was bioinformatically predicted using Epitope mapping software's and the predicted epitopes were expressed in a prokaryotic expression vector. The antigen was injected into the animal model (mice BALB/c) and some indicators including IgG1, IgG2a, IgA, IFN-γ, and IL 5 were measured.
Results:
The immunogenicity studies in animal models by measuring serum antibodies (IgG1, IgG2a, and IgA) and cytokines (IFN-γ and IL5) revealed that the rFlaA induces a proper immune response in animal models.
Conclusion:
The recombinant FlaA protein is antigenic and immunogenic. Therefore, it might be used in order to design of specific diagnostic kits and recombinant vaccines against H. pylori.
Insights
Recombinant FlaA protein from Helicobacter pylori is antigenic and immunogenic, showing potential for developing diagnostic kits and vaccines against this common pathogen.
Area of Science:
- Microbiology
- Immunology
- Molecular Biology
Background:
- Helicobacter pylori is a prevalent human pathogen affecting over half the global population.
- Bacterial motility, particularly flagellar proteins like FlaA, plays a crucial role in H. pylori colonization and invasion.
- Understanding FlaA's antigenicity and immunogenicity is key to developing effective countermeasures.
Purpose of the Study:
- To clone, express, and purify specific epitopes of the FlaA protein.
- To evaluate the antigenicity and immunogenicity of the recombinant FlaA protein.
- To explore the potential of FlaA epitopes in diagnostic and vaccine development.
Main Methods:
- Bioinformatic prediction of antigenic regions within the flaA gene using epitope mapping software.
- Expression of predicted epitopes in a prokaryotic expression vector.
- Immunization of BALB/c mice with the recombinant antigen and measurement of immune responses (IgG1, IgG2a, IgA, IFN-γ, IL-5).
Main Results:
- The recombinant FlaA (rFlaA) protein was successfully expressed and purified.
- Immunogenicity studies in mice demonstrated that rFlaA induces a significant immune response.
- Analysis of serum antibodies (IgG1, IgG2a, IgA) and cytokines (IFN-γ, IL-5) confirmed the immunogenic potential of rFlaA.
Conclusions:
- The recombinant FlaA protein is confirmed to be both antigenic and immunogenic.
- These findings suggest that rFlaA holds promise for the development of targeted diagnostic kits.
- rFlaA represents a potential candidate for the creation of novel recombinant vaccines against H. pylori infections.
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