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Apatinib has anti-tumor effects and induces autophagy in colon cancer cells

Wu Lu1,2, He Ke3,4, Ding Qianshan1

  • 1Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.

Iranian Journal of Basic Medical Sciences
|November 1, 2017
PubMed
Abstract

Insights

Apatinib inhibits colon cancer cell proliferation, migration, and invasion. This drug also induces apoptosis and regulates autophagy, offering potential as a colon cancer therapeutic.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Apatinib is approved for gastric cancer treatment.
  • Its role in colon cancer is not well-understood.

Purpose of the Study:

  • Investigate apatinib's effects on colon cancer cell biological functions.
  • Elucidate the underlying mechanisms of action.

Main Methods:

  • Assessed cell viability, migration, and invasion.
  • Analyzed apoptosis and cell cycle via flow cytometry.
  • Examined autophagy-related proteins and pathways.

Main Results:

  • Apatinib (20, 40 μM) inhibited proliferation, migration, and invasion.
  • Apoptosis increased with higher apatinib concentrations.
  • Apatinib altered cell cycle distribution and inhibited the AKT-mTOR pathway.
  • Increased LC3-II expression indicated autophagy regulation.

Conclusions:

  • Apatinib significantly suppresses colon cancer cell malignant phenotypes.
  • Apatinib's mechanism involves autophagy regulation.
Keywords:
ApatinibApoptosisAutophagyColon cancerMigrationmTOR

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