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VZV Replication Assays
Samantha J Griffiths1, Jürgen Haas1
1Division of Pathway and Infection Medicine, University of Edinburgh, Edinburgh, UK.
Bio-Protocol
|November 1, 2017
Summary
This study presents a Varicella zoster virus (VZV) replication assay using a VZV-GFP reporter virus. The assay enables researchers to investigate cellular gene effects on VZV replication and pathogenesis.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Varicella zoster virus (VZV) causes chickenpox and shingles.
- VZV replication mechanisms and pathogenesis are poorly understood due to its cell-associated nature and limited host range.
- Further research is needed to elucidate VZV-host interactions.
Purpose of the Study:
- To describe a basic Varicella zoster virus (VZV) replication assay.
- To establish a VZV-GFP reporter virus system for studying VZV replication.
- To provide a method for investigating cellular gene functions in VZV replication.
Main Methods:
- Utilized a recombinant VZV expressing Green Fluorescent Protein (GFP) as a reporter.
- Prepared VZV inoculum as infected cells due to VZV's cell-associated nature.
- Combined the VZV-GFP reporter system with siRNA gene depletion and cDNA overexpression techniques.
Main Results:
- Developed a functional VZV replication assay using infected cells as inoculum.
- Demonstrated the utility of the VZV-GFP reporter system for assessing virus replication.
- Established a framework for high-throughput screening of cellular genes affecting VZV replication.
Conclusions:
- The described VZV replication assay provides a valuable tool for VZV research.
- This method facilitates the study of cellular and viral factors influencing VZV pathogenesis.
- Future studies can utilize this assay to identify novel antiviral targets.
