Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes02:31

Replication in Eukaryotes

206.1K
Overview
206.1K
Replication in Prokaryotes02:35

Replication in Prokaryotes

99.7K
Overview
99.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impaired suppressive effect of FoxP3 regulatory T cells on B cells in multiple sclerosis.

Journal of neuroinflammation·2026
Same author

RNA interference screen against SARS-CoV-2 identifies proviral vesicular transport factors involved in release.

The Journal of general virology·2026
Same author

Twin study identifies early immunological and metabolic dysregulation of CD8<sup>+</sup> T cells in multiple sclerosis.

Science immunology·2024
Same author

Single cell transcriptomics of cerebrospinal fluid cells from patients with recent-onset narcolepsy.

Journal of autoimmunity·2024
Same author

Multiple sclerosis endophenotypes identified by high-dimensional blood signatures are associated with distinct disease trajectories.

Science translational medicine·2024
Same author

SLE serum induces altered goblet cell differentiation and leakiness in human intestinal organoids.

EMBO molecular medicine·2024
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
11:19

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Published on: May 4, 2015

11.8K

VZV Replication Assays.

Samantha J Griffiths1, Jürgen Haas1

  • 1Division of Pathway and Infection Medicine, University of Edinburgh, Edinburgh, UK.

Bio-Protocol
|November 1, 2017
PubMed
Summary
This summary is machine-generated.

This study presents a Varicella zoster virus (VZV) replication assay using a VZV-GFP reporter virus. The assay enables researchers to investigate cellular gene effects on VZV replication and pathogenesis.

More Related Videos

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
10:16

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

4.2K
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
14:23

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Published on: August 31, 2014

16.2K

Related Experiment Videos

Last Updated: Feb 19, 2026

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
11:19

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Published on: May 4, 2015

11.8K
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
10:16

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

4.2K
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
14:23

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Published on: August 31, 2014

16.2K

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Varicella zoster virus (VZV) causes chickenpox and shingles.
  • VZV replication mechanisms and pathogenesis are poorly understood due to its cell-associated nature and limited host range.
  • Further research is needed to elucidate VZV-host interactions.

Purpose of the Study:

  • To describe a basic Varicella zoster virus (VZV) replication assay.
  • To establish a VZV-GFP reporter virus system for studying VZV replication.
  • To provide a method for investigating cellular gene functions in VZV replication.

Main Methods:

  • Utilized a recombinant VZV expressing Green Fluorescent Protein (GFP) as a reporter.
  • Prepared VZV inoculum as infected cells due to VZV's cell-associated nature.
  • Combined the VZV-GFP reporter system with siRNA gene depletion and cDNA overexpression techniques.

Main Results:

  • Developed a functional VZV replication assay using infected cells as inoculum.
  • Demonstrated the utility of the VZV-GFP reporter system for assessing virus replication.
  • Established a framework for high-throughput screening of cellular genes affecting VZV replication.

Conclusions:

  • The described VZV replication assay provides a valuable tool for VZV research.
  • This method facilitates the study of cellular and viral factors influencing VZV pathogenesis.
  • Future studies can utilize this assay to identify novel antiviral targets.