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Rapid recovery of high content phytosterols from corn silk
Haiyan Zhang1, Xiaowan Cao1, Yong Liu2
1College of Life Science, Henan University, Dongjing Street, Jinming District, Kaifeng, Henan province, 475004, People's Republic of China.
Background:
Phytosterols have important physiological and officinal function.
Methods:
An efficient ultrasonic assisted extraction, purification and crystallization procedure of phytosterols was established from corn silk for the first time.
Results:
The orthogonal test was applied to optimize the process parameters and a maximum phytosterols recovery as high as 10.5886 mg/g was achieved by ultrasonic treatment for 55 min with liquid-solid ratio of 12:1 at 35 °C, 220 w. The ultrasonic extraction temperature (T, °C) has the most significant effect on extraction yield of phytosterols. An orthogonal crystallization test was performed and the optimal conditions [crystallization temperature of 8 °C, time of 12 h and solid-liquid ratio of 1:1 (g/ml)] afforded maximum phytosterols purity of 92.76 ± 0.43%.
Conclusions:
An efficient extraction and crystallization procedure was established.
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