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Co-localization of IGF-II receptors, IL-1 receptors and Thy 1.1 in rat brain
J M Hill1, M A Lesniak, C B Pert
1Section on Brain Biochemistry, National Institute of Mental Health, NIH, Bethesda, MD 20892.
The distribution of 125I-IGF-II and 125I-IL-1 binding to rat brain sections was determined with autoradiography, Thy 1.1 with radioimmunocytochemistry. The pattern of distribution of all three was found to be identical and almost the same as the pattern achieved by a Nissl stain. In preliminary studies excess IGF-II was shown to inhibit the binding of radiolabeled IGF-II and IL-1 but not anti-Thy 1.1. Similarly, IL-1 inhibited the binding of radiolabeled IL-1 and IGF-II but not anti-Thy 1.1. Anti-Thy 1.1 inhibited the binding of IGF-II and IL-1, but was not inhibited by either IGF-II or IL-1. The distribution data from this study indicates the IGF-II receptors, IL-1 receptors and Thy 1.1 are present on almost all neurons in the brain. The preliminary competition studies suggest that these factors may, under some conditions, function in a common biochemical cellular process to regulate each other's functions. Both IGF-II and IL-1 are growth factors and Thy 1.1 may play a role in synapse formation and cell adhesion. Perhaps, among other things, these growth factors and Thy 1.1 function in the development and maintenance of intercellular contacts within the brain.
The distribution of 125I-IGF-II and 125I-IL-1 binding to rat brain sections was determined with autoradiography, Thy 1.1 with radioimmunocytochemistry. The pattern of distribution of all three was found to be identical and almost the same as the pattern achieved by a Nissl stain. In preliminary studies excess IGF-II was shown to inhibit the binding of radiolabeled IGF-II and IL-1 but not anti-Thy 1.1. Similarly, IL-1 inhibited the binding of radiolabeled IL-1 and IGF-II but not anti-Thy 1.1. Anti-Thy 1.1 inhibited the binding of IGF-II and IL-1, but was not inhibited by either IGF-II or IL-1. The distribution data from this study indicates the IGF-II receptors, IL-1 receptors and Thy 1.1 are present on almost all neurons in the brain. The preliminary competition studies suggest that these factors may, under some conditions, function in a common biochemical cellular process to regulate each other's functions. Both IGF-II and IL-1 are growth factors and Thy 1.1 may play a role in synapse formation and cell adhesion. Perhaps, among other things, these growth factors and Thy 1.1 function in the development and maintenance of intercellular contacts within the brain.