Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Vicennial metagenomic time series unveils evolutionary dynamics of giant viruses in a freshwater ecosystem.

Nature communications·2026

Same author

Hunting for new lineages and mechanisms of life.

Nature microbiology·2026

Same author

Complex viral interactions revealed for the harmful bloom-forming dinoflagellate <i>Karenia brevis</i>.

ISME communications·2026

Same author

Draft genome sequence of <i>Paenibacillus turicensis</i> DSM 14349<sup>T</sup> from cerebrospinal fluid of a patient in Switzerland.

Microbiology resource announcements·2026

Same author

Predicting phage-host specificity.

Nature reviews. Microbiology·2026

Same author

Global metagenomics reveals plastid diversity and unexplored algal lineages.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Feb 19, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

The trajectory of microbial single-cell sequencing.

Tanja Woyke¹, Devin F R Doud¹, Frederik Schulz¹

¹DOE Joint Genome Institute, Walnut Creek, California, USA.

|November 1, 2017

Summary

This summary is machine-generated.

Single-cell genome sequencing is revolutionizing microbial community studies by complementing metagenomics. This technique reveals new bacterial and archaeal life, offering unique advantages for future research.

More Related Videos

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Related Experiment Videos

Last Updated: Feb 19, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Single-cell genome sequencing has become a standard technique for analyzing microbial communities.
It complements traditional shotgun metagenomics, offering deeper insights into microbial life.
Environmental samples range from deep-sea vents to insect guts.

Purpose of the Study:

To review the technical aspects and challenges of single-cell genome sequencing.
To highlight scientific advancements enabled by this technology.
To illustrate the advantages of single-cell genomics over metagenomics.

Main Methods:

Review of current literature on single-cell genome sequencing techniques.
Analysis of case studies demonstrating the application of single-cell genomics.
Comparative analysis of single-cell genomics and metagenomics.

Main Results:

Single-cell genomics has expanded our understanding of bacterial and archaeal diversity.
It provides a powerful tool for reconstructing genomes from unculturable microbes.
New branches and leaves have been added to the genomic tree of life.

Conclusions:

Single-cell genome sequencing offers unique advantages over metagenomics for microbial community studies.
This technology is crucial for exploring microbial dark matter and understanding ecosystem functions.
Future applications hold significant promise for microbiology and biotechnology.