Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.5K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuous-Flow Plasma Synthesis of Single-Atom Au<sub>1</sub>/CeO<sub>2</sub>-Fe for Wide-Temperature-Window Water-Gas Shift Reaction.

JACS Au·2026
Same author

Dietary and metabolic reprogramming alleviates neurodegeneration: a review of mechanisms and clinical implications.

Frontiers in nutrition·2026
Same author

Tuning TiO<sub>2</sub>/Co<sub>3</sub>O<sub>4</sub> Nano-interface for bridging photo- and plasma-catalytic reactive oxygen species generation.

Journal of colloid and interface science·2026
Same author

High-Recovery Submicron Particle Detection Enabled by Weakly Deformed Thread Flow.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Visualizing intraorganellar ultrastructures, dynamics, and interactions with open-access background-free Lock-in-SIM.

Nature communications·2025
Same author

Solution Plasma Synthesis of High-Entropy Alloy Nanoparticles with Self-Limiting Oxidation for Photothermal CO<sub>2</sub> Reduction.

Advanced materials (Deerfield Beach, Fla.)·2025

Related Experiment Video

Updated: Feb 19, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.4K

High-contrast wide-field evanescent wave illuminated subdiffraction imaging.

Chenlei Pang, Xiaowei Liu, Minghua Zhuge

    Optics Letters
    |November 1, 2017
    PubMed
    Summary

    This study demonstrates high-contrast, wide-field subdiffraction imaging by controlling light-matter interactions. The technique achieves nanoscale resolution over large areas, improving image quality and field of view.

    More Related Videos

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    16.2K
    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
    08:13

    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

    Published on: April 8, 2019

    18.1K

    Related Experiment Videos

    Last Updated: Feb 19, 2026

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.4K
    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    16.2K
    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
    08:13

    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

    Published on: April 8, 2019

    18.1K

    Area of Science:

    • Optics and Photonics
    • Nanotechnology
    • Microscopy

    Background:

    • Subdiffraction imaging offers higher resolution than conventional microscopy.
    • Evanescent wave illumination is a promising technique for nanoscale imaging.
    • Achieving high contrast and large fields of view simultaneously remains a challenge.

    Purpose of the Study:

    • To develop a method for high-contrast, wide-field evanescent wave illuminated subdiffraction imaging.
    • To leverage nanoscale light-matter interactions for improved imaging performance.
    • To demonstrate the technique's capability for high resolution over large areas.

    Main Methods:

    • Controlling nanoscale light-matter interactions for evanescent wave illumination.
    • Exploiting polarization selectivity and film quality dependence in light coupling and propagation.
    • Implementing wide-field imaging techniques.

    Main Results:

    • Achieved high contrast-to-noise ratio (CNR) subdiffraction imaging.
    • Demonstrated a lateral resolution of 122 nm.
    • Obtained a field of view (FOV) of thousands of micrometers square.

    Conclusions:

    • The developed method enables high-contrast, wide-field subdiffraction imaging.
    • Controlling light-matter interactions is crucial for optimizing CNR and FOV.
    • This technique significantly advances nanoscale imaging capabilities.