Evaluation of right heart function in a rat model using modified echocardiographic views

Area of Science:

• Cardiovascular Imaging
• Veterinary Cardiology
• Animal Models

Background:

• Echocardiography is crucial for assessing cardiac function in animal research.
• Standard views may not fully capture right ventricular (RV) morphology and function.
• Advanced imaging techniques are needed for detailed RV assessment in rodents.

Purpose of the Study:

• To evaluate modified parasternal mid right-ventricular (MRV) and right ventricle outflow tract (RVOT) views for assessing RV size and function in rats.
• To determine the suitability of 2D-strain analysis for evaluating RV contractility.
• To correlate echocardiographic measurements with necropsy findings.

Main Methods:

• Transthoracic echocardiography was performed on 15 WKY rats.
• Modified MRV and RVOT views were used to measure RV dimensions and areas.
• 2D-strain analysis was applied to both ventricles.
• RV chamber volume was quantified using agarose gel, and RV free wall was weighed.

Main Results:

• RV mid-ventricular (R1) and RVOT (R2) dimensions were measured (0.42±0.07cm and 0.34±0.06cm).
• MRV and RVOT chamber areas correlated with agarose gel mass.
• Doppler-derived RV stroke volume and cardiac output were recorded (0.32±0.08ml and 110±27 ml/min).
• RV free wall contractility was assessed using 2D-strain analysis.

Conclusions:

• Modified MRV and RVOT views provide a detailed assessment of the rodent right ventricle.
• 2D-strain analysis is potentially feasible for evaluating RV free wall contractility in this model.
• These methods enhance echocardiographic evaluation of RV in small animal research.