Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A minibead method for detection of membrane lectins.

T Matsuoka1, M Tavassoli

  • 1Veterans Administration Medical Center, Jackson, Mississippi.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|January 1, 1989
PubMed
Summary

A new method uses sugar-coated minibeads to identify specific membrane lectins on cells. This technique allows for the visualization and differentiation of lectin-bearing cells in complex biological samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of Neospora caninum as an etiologic agent of animal abortion in Kurdistan Region of Iraq.

Polish journal of veterinary sciences·2023
Same author

Phylogenetic Diversity of <i>Dermanyssus gallinae</i> (Dermanyssidae) based on Mitochondrial Cytochrome Oxidase-1 Gene Sequence Collected from Different Bird Species in Iran.

Archives of Razi Institute·2023
Same author

Molecular Characterization and Phylogenetic Analysis of Pathogenic Theileria spp. Isolated from Cattle and Sheep based on Cytochrome b Gene in Iran.

Archives of Razi Institute·2021
Same author

Histopathological changes and oxidative damage in hepatic tissue of rats experimentally infected with Babesia bigemina.

Polish journal of veterinary sciences·2018
Same author

Clinical immunotherapeutic approaches for the treatment of head and neck cancer.

International journal of oral and maxillofacial surgery·2018
Same author

Effects of Essential Oils Combination on Sporulation of Turkey (Meleagris gallopavo) Eimeria Oocysts.

Archives of Razi Institute·2018

Area of Science:

  • Cell Biology
  • Biochemistry
  • Materials Science

Background:

  • Membrane lectins play crucial roles in various biological processes.
  • Existing methods for lectin detection are limited to homogeneous cell populations.
  • There is a need for techniques to identify lectin-expressing cells within heterogeneous mixtures.

Purpose of the Study:

  • To develop a novel method for the morphological identification of lectin-bearing cells in heterogeneous cell populations.
  • To enable specific and visual detection of membrane lectins.
  • To allow for semiquantification of lectin binding.

Main Methods:

  • Amide-modified latex minibeads (0.345 or 0.532 micron) were activated with glutaraldehyde.
  • Minibeads were covalently linked to p-aminophenyl derivatives of various sugars, creating specific probes.

Related Experiment Videos

  • Probes were incubated with cell systems expressing known membrane lectins (e.g., galactosyl receptors in hepatocytes, mannosyl receptors in macrophages).
  • Binding was visualized using scanning electron microscopy.
  • Specificity was confirmed by inhibition with excess soluble sugar.
  • Differentiation of cell types was achieved using differently sized probes.

    • Main Results:

    • The developed probe successfully bound to known membrane lectins on specific cell types.
    • Binding was specific, as demonstrated by inhibition with excess soluble sugars.
    • Scanning electron microscopy allowed for clear visualization of lectin-cell interactions.
    • The method enabled the segregation of two different cell types using distinct probes.
    • The technique allows for semiquantification of lectin binding.

    Conclusions:

    • This novel minibead-based method enables specific and morphological identification of membrane lectins on cells within heterogeneous populations.
    • The technique offers a valuable tool for studying cell-cell interactions and receptor expression in complex biological systems.
    • The method's specificity and semiquantification capabilities enhance its utility in biological research.