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Molecular Insights into Antimicrobial Resistance Traits of Multidrug Resistant Enteric Pathogens isolated from India

Pawan Kumar1, Satyabrata Bag1, Tarini Shankar Ghosh1

  • 1Molecular Genetics Laboratory, Centre for Human Microbial Ecology, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, 121001, India.

Scientific Reports
|November 2, 2017
PubMed

Insights

Antimicrobial resistance (AMR) in Indian enteric pathogens is widespread, with most isolates resistant to multiple antibiotics. Mobile genetic elements drive the spread of this resistance, complicating treatment strategies.

Area of Science:

  • Microbiology
  • Genomics
  • Public Health

Background:

  • Antimicrobial resistance (AMR) in Gram-negative bacteria poses a significant global health threat.
  • Limited data exists on AMR patterns in India, a critical region for this crisis.
  • Enteric pathogens are a major source of AMR infections.

Purpose of the Study:

  • To investigate the prevalence and patterns of AMR in enteric pathogens in India.
  • To analyze the whole genome sequences of resistant isolates to understand the genetic basis of AMR.
  • To identify mobile genetic elements contributing to the spread of AMR.

Main Methods:

  • Isolation and antimicrobial susceptibility testing of 654 enteric pathogens.
  • Whole genome sequencing of six extensively drug-resistant isolates.
  • Analysis of mobile genetic elements and their association with resistance genes.

Main Results:

  • Approximately 97% of isolates showed resistance to at least two antibiotics.
  • About 24% of isolates were resistant to 10 or more antibiotics, and 3% to 15 or more.
  • Whole genome sequencing revealed mobile genetic elements linked to resistance traits, facilitating horizontal gene transfer.

Conclusions:

  • Widespread AMR in Indian enteric pathogens necessitates urgent public health interventions.
  • Mobile genetic elements play a crucial role in the dissemination of AMR.
  • Understanding the genetics of AMR is vital for developing effective strategies to combat this growing threat.

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