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Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering.

Yang Liu¹, Ye Liu¹, Meng Wang¹

¹Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.

Frontiers in Microbiology

|November 2, 2017

Summary

Small molecule biosensors are crucial for metabolic engineering, enabling precise control over microbial cell factories. This review focuses on optimizing transcription factor-based biosensors for bio-based economy applications.

Keywords:

industrial application metabolic engineering optimization strategy small molecule biosensor synthetic biology transcription factor

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Area of Science:

Synthetic biology
Metabolic engineering
Bio-based economy

Background:

Synthetic biology and metabolic engineering advance the bio-based economy, producing fuels, chemicals, and drugs from renewable resources.
Genome-scale modeling, pathway assembly, and genome engineering enable the design of microbial cell factories.
A key bottleneck in metabolic engineering is the lack of precise control over metabolite concentration-related phenotypes.

Purpose of the Study:

To review recent advances in the design, optimization, and application of small molecule biosensors in metabolic engineering.
To highlight optimization strategies for transcription factor (TF)-based biosensors.

Main Methods:

Review of literature on small molecule biosensor development.
Focus on transcription factor (TF)-based biosensor optimization strategies.

Main Results:

Small molecule biosensors couple metabolite concentrations to measurable outputs, addressing a critical bottleneck.
Advances in biosensor design and optimization enhance metabolic engineering capabilities.
Transcription factor (TF)-based biosensors show significant promise for precise metabolic control.

Conclusions:

Small molecule biosensors are essential tools for advancing metabolic engineering and the bio-based economy.
Optimization of biosensors, particularly TF-based ones, is key to unlocking their full potential.
Further development of biosensors will enable more sophisticated microbial cell factory designs and applications.